Figure 4. RapGEF1 silencing increases SM22α mRNA and eliminates elevated Pi-induced SM22α mRNA inhibition.
A) SM22α mRNA expression was quantified at day 2, 3, and 4 post-transfection with either siNegative or siRapGEF1. B) VSMCs were treated with either siNegative or siRapGEF1 and incubated for 4 days in 1.0mM Pi or 2.6mM Pi media and SM22α mRNA was quantified. (two-way ANOVA post-hoc Sidak in A and B: ns = p > 0.05, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001)
