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. 2021 Aug 2;3(1):vdab103. doi: 10.1093/noajnl/vdab103

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© The Author(s) 2021. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — In vitro effect of IDH mutant-specific inhibitors on IDHmt glioma cultures. (A) Percentage of D2-HG in IDHmt (N = 6) and IDHwt (N = 2) cell cultures treated with increasing concentrations of IDH-mutant specific inhibitor AGI-5198 compared to DMSO-controls. Each datapoint represents a single measurement. (B) Mean average survival of IDHmt (N = 6) and IDHwt (N = 2) cultures treated with increasing concentrations of BAY-1438032 or AGI-5198. Data are presented as mean percentage survival ± SD. Each culture was tested in three technical replicates. (C) Mean doubling times of IDHmt (N = 6) and IDHwt (N = 2) cell cultures, cultured with and without 10 µM IDH mutant-specific inhibitor AGI-5198. Error bars represent standard deviations over three consecutive passages. (*P < .05, paired T-test). Three technical replicates were used for each culture and each time point. (D) Heatmap showing unsupervised cluster analysis of RNA expression of IDHmt (N = 8) and IDHwt (N = 4) glioma cultures, cultured with and without IDH mutant-specific inhibitor AGI-5198 for seven days. Unsupervised clustering was performed on the 1000 most variably expressed genes. The heatmap was scaled by row.