Figure 2.

Peripheral immune cell infiltration in ischemic brains at 3 and 7 days after stroke. (A) Representative dot plots depict the gating strategy used to differentiate the microglia (CD45^intermediateCD11b⁺), peripheral myeloid cells (CD45^highCD11b⁺) (pMyeloid), and lymphocytes (CD45^highCD11b⁻). In pMyeloid cells, monocytes are further gated as CD45^highCD11b⁺Ly6C⁺Ly6G⁻, and neutrophils as CD45^highCD11b⁺Ly6G⁺ Ly6C⁻. Absolute counts of infiltrating monocytes, neutrophils, and lymphocytes at 3d (B) and 7d (C) after stroke were shown. N=4–5 sham and 6–8 stroke animals per group; *P < 0.05.