Fig. 3. Assessment of mechanical property and mineralization.
(A) The compressive modulus of cell-laden scaffolds before and after 28 days of culture in OM. N = 4, data analyzed by unpaired t-test. P values were labeled. (B) Representative stress-strain curves of the D28 samples. (C, D) Alizarin Red staining (C) and von Kossa staining (D) images showing mineralization in the constructs after 28 days of culture in OM. The dotted circles in D depicts the outline of the entire section. Scale bars = 1 mm (top row) or 50 μm (bottom row). (E) Confocal images of calcein green-stained calcium minerals in the D28 samples. Scale bar = 100 μm.