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(A) Timeline of the in vivo subcutaneous implantation experiment. (B) Representative μCT images showing bone formation in the implanted constructs on day 28 post-surgery. Scale bar = 1 mm. (C) Quantitative analyses of bone volume and bone mineral density (BMD). N = 3; analyzed by one-way-ANOVA. P values were labeled. (D) Alizarin Red staining and (E) IF analysis of the 28 day implants. Scale bars = 50 μm.
