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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Neuropharmacology. 2021 Jul 31;197:108737. doi: 10.1016/j.neuropharm.2021.108737

Figure 1. # 11a inhibits AEP activity in primary neurons.

Figure 1.

(A) Chemical Structure of the top 6 candidates.

(B) IC50 values of the compounds toward AEP.

(C) CP24 (#11a) binds to active AEP recombinant protein in Biacore assay. Active AEP recombinant protein was immobilized on Sam5 Chip.

(D) Immunoblotting analysis of AEP in vitro.

(E) Enzymatic activity of AEP was determined using an AEP fluorogenic substrate.

(F) Microscopy images of neurons exposed to different concentration of #11a, followed by incubation with LE28. Scale bar, 50μm.

(G) Quantification of LE28 fluorescent intensity in primary neurons.