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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Neuropharmacology. 2021 Jul 31;197:108737. doi: 10.1016/j.neuropharm.2021.108737

Figure 4. #11a, CF3CN and their mixture block AEP in the brain of 3xTg mice.

Figure 4.

(A) Immunoblotting analysis of AEP, the p-TrkB and their downstream in vitro.

(B) Quantitative analysis of p-TrkB/TrkB, p-Akt/Akt and p-ERK/ERK in the brain in 3xTg mice. (n=3, one-way ANOVA)

(C) Enzymatic activity of AEP was determined using a fluorogenic substrate. (n=3, one-way ANOVA) (D) Imaging of 3xTg mice by using IVIS 100 after injection with LE28.

(E) Relationship between quantified LE28 signal intensity and drug concentrations in 3xTg mice brain. (n=3, one-way ANOVA)

(F) hAβ 40 and hAβ 42 levels were determined by ELISA. Both of hAβ 40 and hAβ 42 concentrations were reduced by #11a treatment. (n=3, one-way ANOVA)

(G) pro-inflammatory cytokines were determined by ELISA. The level of IL-6 was diminished by #11a and CF3CN treatments. (n=3, one-way ANOVA)

(Data are shown as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001).