Fig. 4.
Results of alkylation studies of STAT3 by iodoacetamide, NEM and TTI-101. Schematic depicting chemistry of possible alkylation of STAT3 by iodoacetamide the results of LC-MS chromatograms of STAT3 peptides demonstrating alkylated peptides, as predicted from the chemistry. Schematic depicting chemistry of possible alkylation of STAT3 by NEM the results of LC-MS chromatograms of STAT3 peptides demonstrating alkylated peptides, as predicted from the chemistry. Schematic depicting the chemistry of possible alkylation of STAT3 by TTI-101. The LC-MS chromatograms of STAT3 peptides, that, shows no MRM signal for predicted peptide adducts, indicating that no peptides were alkylated.