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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Biomaterials. 2021 Aug 25;277:121097. doi: 10.1016/j.biomaterials.2021.121097

Figure 2: BAG3 knockdown desensitizes C2C12 cells to matrix stiffness.

Figure 2:

A) Schematic illustration of the SPAAC in situ stiffening reaction, which takes place upon light irradiation if the initial formulation contains excess DBCO groups. B) Young’s modulus (E’) measurement of a SPAAC hydrogel with rheology. Hydrogel was composed of a DBCO:N3 stoichiometric ratio of 4:1, and irradiated with 365 nm light with 5 mW/cm2 intensity for 120 s with 2 mM LAP. Normalized YAP/TAZ Nuc:Cyto ratio of (C) shNT and (D) shBAG3 cells cultured on stiff (E’= 32 kPa), soft (E’ = 4 kPa), and in situ stiffened (E’ = 4 → 32 kPa) hydrogels. In situ stiffening was carried out 24 h after seeding. *** < 0.001 in a one-way ANOVA test comparing means obtained on E’ = 4 and 32 kPa hydrogels, and ### < 0.001 in a one-way ANOVA test comparing means w.r.t to 24 h post-seeding timepoint. n > 155 cells, 3 independent hydrogels. E) Proposed mechanism for BAG3 regulation of YAP/TAZ mechanotransduction.