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. 2021 Sep 28;11:19202. doi: 10.1038/s41598-021-98738-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondrial staining on Aedes fluviatilis cell lines. Mitochondrial staining on Aedes fluviatilis cell lines wAflu1 (Wolb+) and wAflu1.tet (Wolb−) was detected using MitoTracker specific staining (A, C), respectively. Mitochondria change distribution in presence of Wolbachia showing more diffuse marking in wAflu1 (B) than wAflu1.tet (D) cell culture. The (B′, D′) represent the analysis of the cell indicated by an asterisk in (B, D) and magenta region is the binarized signal used for calculating the area (E). The green lines are the morphological skeleton and yellow dots represent the end points of the skeleton and the blue dots represent the junctions. Mitochondrial mass was higher in wAflu1 cells than in wAflu1.tet inferred by mitochondrial footprint and summed branch length mean (E). Scale Bar: 20 µm.