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. 2021 Sep 28;11:19202. doi: 10.1038/s41598-021-98738-7

Figure 7.

Figure 7

Innate immunity was activated by heat-killed Gram-negative (Enterobacter cloacae) bacteria.wAflu1 and wAflu1.tet cell cultures were challenged for 24 h with 106 or 107 heat-killed Enterobacter cloacae cells, then used for relative mRNA quantification of Relish 1 (A), Relish2 (B), defensin (C) and cecropin (D). Schematic representation (Created with BioRender.com) summarizing the different responses of A. fluviatilis cells to heat-killed Gram-negative E. cloacae (E). mRNA levels are expressed as mean ± SD (n = 3). Quantitative PCRs were statistically analyzed by ANOVA followed by Dunnett’s multiple comparison test. Asterisks indicate significant differences (**p < 0.01; ***p < 0.001).