Figure 1 -. EpiP is a dose-dependent hypnotic agent.

A. WT mice demonstrated a dose-dependent decrease in time to LORR onset with increasing EpiP dose (one way ANOVA F_(4,34) = 9.494, p < 0.001). This effect was prominently seen in doses higher than 50 mg/kg. Specifically, we found a significant decrease in time to LORR onset from 50 mg/kg to 65 mg/kg, 75 mg/kg, 100 mg/kg, and 120 mg/kg, (p <0.001, <0.001, <0.001, and <0.001, respectively). B. LORR duration exhibited a dose-dependent response to EpiP (one way ANOVA F_(4,34) = 10.000, p < 0.001). Statistical analysis yielded between 50 mg/kg and 100 mg/kg as well as 120 mg/kg (p = 0.003, <0.0001); between 65 mg/kg and 120 mg/kg (p = 0.002); and finally 75 mg/kg and 120 mg/kg (p = 0.006). C. Average duration of LORR is plotted against the injected dose of EpiP (same data as panel B of this figure). Solid red line is best fit of the data points using Hill-Langmuir equation yielding estimated ED₅₀ of 72.53 ± 4.00 mg/kg and slope n of 4.05 ± 0.98. Fit was constrained to the maximal duration of 56.91 minutes achieved with dose of 120 mg/kg. *vs 50 mg/kg EpiP, #vs 65 mg/kg EpiP, +vs 75 mg/kg EpiP