Figure 8 -. EpiP induces dose-dependent hypnosis in CaV3.3 KO mice that is not significantly different from WT mice.
A. Increasing doses of EpiP caused a decreasing time to LORR in a dose-dependent fashion (one-way ANOVA F(3,18) = 13.580, p < 0.001). Post-hoc analysis illustrated significance between 50 mg/kg and both 75 mg/kg (p = 0.005) as well as 100 mg/kg (p < 0.001); and 65 mg/kg and 100 mg/kg (p = 0.003). B. CaV3.3 KO mice demonstrated a dose-dependent hypnosis duration in response to EpiP (one-way ANOVA F(3,28) = 7.906, p < 0.001). Bonferroni’s multiple comparisons specifically showed significant differences between 50 mg/kg and 100 mg/kg (p < 0.001); and between 65 mg/kg and 100 mg/kg (p = 0.006). C. The CaV3.3 KO mice exhibited no difference in LORR onset from WT mice (Interaction F(3,44) = 4.168, p = 0.011; Dose F(3,44) = 24.440, p < 0.001, Genotype F(1,44) = 1.556, p = 0.219). D. We did not find a significant difference in overall LORR duration between CaV3.3 KO and WT mice. (Interaction F(3,65) = 2.230, p = 0.093; Dose F(3,65) = 12.730, p < 0.001, Genotype F(1,65) = 3.616, p = 0.062). Despite the overall insignificant finding, there appears to be a trend indicating that CaV3.3 KO mice showed longer LORR duration than WT mice at the highest dose. The WT data shown here is the same as those from Figure 1. *vs 50 mg/kg EpiP, #vs 65 mg/kg EpiP, +vs 75 mg/kg EpiP