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. 2021 Sep 28;11:19214. doi: 10.1038/s41598-021-98768-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of acute limonene administration on zebrafish behaviour assessed by the open field test. (A) Group heatmaps showing a coloured representation of the combined mean location of each group of fish across all trials. Average time spent in the center (B), transition (C), and thigmotaxis (D) zone during the open field test. Fish locomotion was quantified in the open field test by measuring distance moved (E), meandering (F), time spent immobile (G), and time spent highly mobile (H). All data are presented as mean ± S.E.M. Significant differences between controls and limonene treated groups are indicated by *(P < 0.05), **(P < 0.01), ***(P < 0.001), and ****(P < 0.0001).