Table 1.
Physicochemical properties and diffusivity of PLGA-PEG nanoparticles formulated with biocompatible surfactants. Nanoparticles were characterized in terms of hydrodynamic diameter, mean surface charge (ζ-potential), and the polydispersity index (PDI) by dynamic light scattering at 25°C and pH 7.2 in 10 mM NaCl. All values are reported as mean ± standard error of the mean (SEM) (n=3). Effective diffusion coefficients in rat brain tissue (Db) were extracted at a τ = 0.8 s from nanoparticle trajectories over fifteen videos across three brain slices for each formulation, and compared to theoretical nanoparticle diffusion in artificial cerebrospinal fluid (aCSF) based on the Stokes-Einstein equation and mean particle diameter.
| Polymer/ Surfactant | Number Mean ± SEM (nm) | PDI | ζ-potential ± SEM (mV) | Ensemble Db (x10−4 μm2/s) | DaCSF/ Db | Number of Trajectories |
|---|---|---|---|---|---|---|
| PLGA/ 1% F127 | 68.2 ± 4.0 | 0.20 | −6.28 ± 0.3 | 1.02 | 94,000 | 557 |
| PLGA-PEG/ 1% P80 | 59.6 ± 1.5 | 0.08 | −3.63 ± 0.8 | 4.72 | 23,000 | 568 |
| PLGA-PEG/ 5% F68 | 65.5 ± 4.1 | 0.09 | −4.33 ± 0.4 | 5.98 | 17,000 | 569 |
| PLGA-PEG/ 1% F127 | 59.7 ± 1.3 | 0.08 | −4.06 ± 1.4 | 7.50 | 15,000 | 839 |
| PLGA-PEG/ 5% PVA | 65.3 ± 2.4 | 0.11 | −3.24 ± 0.9 | 8.48 | 12,000 | 339 |
| PLGA-PEG/ DI H2O | 55.0 ± 2.9 | 0.22 | −4.58 ± 0.6 | 12.9 | 9,000 | 590 |
| PLGA-PEG/ 3% CHA | 56.2 ± 3.0 | 0.17 | −6.11 ± 0.6 | 21.7 | 5,000 | 308 |