Figure 5.

Bilayer mesh used in the study design was created by loading FGF into the Gelatin film which was casted onto PCL film showing preparation of bilayer meshes; PCL films were prepared by dissolving 5% polymer in dichloromethane. Dried PCL films were immersed in 10% hexane diamine-isopropanol solution for 1 h at 37 °C for aminolization. PCL films were washed with deionized water for 24 h. Gelatin solution was prepared by dissolving 5% polymer in distilled water and with addition of glutaraldehyde as crosslinker, and FGF. Gelatin solution was poured onto aminolyzed PCL films and let dry at room temperature. A full thickness semicircular defect of 0.5 × 0.5 cm² was created via cutting in the anterior wall of the abdominal esophagus. The defect was repaired with primary anastomosis with the prepared bilayer mesh, using interrupted sutures.