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. 2021 Sep 28;12(10):885. doi: 10.1038/s41419-021-04146-0

Fig. 2. HOTAIRM1 knock-down decreases oncogenic features in glioblastoma cell lines.

Fig. 2

siRNA-mediated knock-down was achieved using siPOOLS (siTOOLs Biotech, Planegg, Germany). A qRT-PCR was performed using TaqMan probes against HOTAIRM1 or phosphoglycerate kinase 1 (PGK1) as a housekeeping control gene. Following HOTAIRM1 knock-down, the four investigated glioma cell lines showed reduced cell viability as determined with the CellTiter-Glo assay (B), reduced invasiveness measured in Boyden chamber assays (C), and, finally, decreased clonogenicity as determined by colony formation assays after seeding cells at a density of 500 (U251MG and LN-18) to 1000 (LN-229 and T98G) cells per 10 cm dish (D). White bars indicate the results of the respective control-transfected cells set to 100%. Filled bars are results obtained with HOTAIRM1 knock-down cells. siControl: cells transfected with non-target siPOOLS; siHOTAIRM1: cells transfected with siPOOLS against HOTAIRM1. Two-way ANOVA was used for statistical analyses; mean ± SEM, ***p < 0.001, **p < 0.01. n = 4 independent experiments for the colony formation assays for T98G cell line, n = 3 independent experiments per cell line and assay.