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. 2021 Sep 28;12(10):885. doi: 10.1038/s41419-021-04146-0

Fig. 3. Knock-down of HOTAIRM1 results in mitochondrial dysfunction and increased reactive oxygen species (ROS).

Fig. 3

A Merged GSEA of RNA sequencing and proteomics data showing overlapping geneset clusters related to mRNA processing/ribosome function, mitochondrial translation, translation and mitochondrial membrane (see supplementary tables 2–5 for the list of the genesets). B Quantification of HEt (general superoxide indicator) and MitoSox (mitochondrial superoxide indicator) staining performed on stable LN-229, SF126, and U87MG HOTAIRM1 knock-down cells and respective controls (n = 3). Shown are ROS levels normalized to control cells. C Results of colony formation assays 21 days post antioxidant NAC treatment in stable LN-229 and LN-18 HOTAIRM1 knock-down and control cells (n = 3). Two-way ANOVA was used for statistical analyses; mean ± SEM, ***p < 0.001, **p < 0.01, *p < 0.05.