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. 2021 Sep 28;12(10):885. doi: 10.1038/s41419-021-04146-0

Fig. 4. HOTAIRM1 knock-down sensitizes glioblastoma cells to radiation in vitro and in vivo.

Fig. 4

A, B, C Representative images and quantification of colony formation assays 21 days post irradiation at indicated doses for stable (A) LN-229, (B) SF126, and (C) LN-18 HOTAIRM1 knock-down (KD) and control cells. Counts were normalized to the corresponding counts in isogenic controls at respective radiation dose (D, E) Kaplan–Meier survival plots of mice harboring either HOTAIRM1 stable knock-down or control transfected LN-229 orthotopic xenografts not treated with radiation (D) and treated with 12 Gy radiation at day 15 (arrow) (E). Gray and black lines represent HOTAIRM1 knock-down and controls, respectively. shControl: non-target shRNA; shHOTAIRM1: shRNA against HOTAIRM1. Two-way ANOVA was used for colony formation statistical analyses and log rank analysis for Kaplan-Meier survival plots; ***p < 0.001, **p < 0.01, *p < 0.05.