Table 2.
Primer sequences used in qRT-PCR analysis.
| Genes | Primers | Primer length | Product length | Tm | GC% |
|---|---|---|---|---|---|
| B-ACTIN |
Forward: 5'TTCGAGCAAGAGATGGCCA3' Reverse: 5' CACAGGACTCCATGCCCAG3' |
19 19 |
151 |
67/56 98/60 |
6/52 2/63 |
| IL7Rα |
Forward: 5'TCATTCATTTCATACACACTGGCT3' Reverse: 5'TCCAAGTCTCCATTTTGAGCATAG3' |
24 24 |
150 |
59/57 30/59 |
|
| NFATc2 |
Forward: 5'AACAAGCATATCCGCACACC3' Reverse: 5'GGTCATATTCATCCGTGGGCT3' |
20 21 |
133 |
30/57 82/59 |
50 4/52 |
| RNF213 |
Forward: 5'TGCTGGGAGACATGGAATGG3' Reverse: 5'GCCACACTGTCCTCTAAGCC3' |
20 20 |
106 |
35/59 60/61 |
55 60 |