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. 2021 Sep 28;11:19265. doi: 10.1038/s41598-021-98657-7

Figure 5.

Figure 5

Diagrammatic workflow of the experiment. Preparation of complete growth media for CCs, optimizing different lipofection reagents, lentivirus production and the relative ratio of transfer, packaging and envelope plasmids, viral titering and purification, concentration, as well as selection method of cells carrying the transgene.