Fig. 3. Effect of PD-1 knockout on abacavir (ABC)-induced activation of T cells in CD4⁺ T cell-depleted HLA-B*57:01 transgenic (Tg) mice.

a and b Representative dot plots depicting effector memory T cells in gated CD8⁺ T cells, classified by the phenotype of CD44 and CD62L expression in the auricular lymph node (LN) from PBS-treated or CD4⁺ T cell-depleted mice. Each group was orally administrated 1% (w/w) ABC or a normal diet (vehicle) for 1 week. Effector memory T cells: CD44^highCD62L^low. Data are representative of three independent experiments. c Percentages of effector memory T cells among CD8⁺ T cells isolated from the auricular LN of 1% (w/w) ABC-fed mice. Each plot represents an individual mouse with the mean ± SEM (N = 3–16); ***p < 0.001, compared with other mice groups; ^#p < 0.05, compared with CD4⁺ T cell-depleted B*57:03-Tg mice or littermates (LMs), one-way ANOVA with Bonferroni’s multiple comparisons correction. Data are a summary of three independent experiments (except the CD4⁺ T cell-depleted B*57:01-Tg/PD-1^−/− mice, serving as the positive control for each independent experiment).