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. 2021 Sep 15;12:730434. doi: 10.3389/fimmu.2021.730434

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Copyright © 2021 Carriquiriborde, Martin Aispuro, Ambrosis, Zurita, Bottero, Gaillard, Castuma, Rudi, Lodeiro and Hozbor

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biofilm formation by B. pertussis Tohama I strain (gray column) and the clinical isolate BpAR106 (black column) on an abiotic surface. Biofilm biomass was quantified at 96 h of growth by staining with crystal violet and absorbance was measurement at OD 595 nm. Each value represents the mean from three independent experiments and the bars indicate standard deviation. Statistically significant differences in Student’s t-test between the clinical isolate and B. pertussis Tohama I biomass absorbance are indicated by * (p < 0.05).