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. 2021 Sep 15;11:727306. doi: 10.3389/fonc.2021.727306

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Copyright © 2021 Mu, Zhu, Dong, Shi, Deng and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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JAK2 or HIF-1 inhibition reduced CALM2’s impact on GC cell proliferation, migration, and invasion. The JAK2 inhibitor LY2784544 (1 μM) and HIF-1 inhibitor SYP-5 (10 μM) were applied to AGS and MKN45 cells with CALM2 overexpression for treatment. (A) Western Blot determined the profile of the JAK2/STAT3/HIF-1/VEGFA signaling axis in AGS and MKN45 cells. (B) The proliferation of those cells was monitored by CCK8. (C) Colony formation assay examined the colony formation of AGS and MKN45 cells. (D, E) Transwell measured the cells’ migration and invasion. (F) EMT-correlated proteins in AGS and MKN45 cells were examined through Western Blot. Statistics were displayed as mean ± SD (n=3). *P < 0.05, **P < 0.01, ***P < 0.001 (vs. the Vector group). NSP > 0.05, &P < 0.05, &&P < 0.01, &&&P < 0.001 (vs. the CALM2 group).