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. 2021 Sep 15;11:699847. doi: 10.3389/fonc.2021.699847

Figure 3.

Figure 3

Gimap5 inhibited migration, invasion and proliferation and promoted apoptosis of lung cancer cells (n = 3). (A) Gimap5 overexpression inhibited the invasion of all three cancer cell lines using Transwell assay. (B) Gimap5 overexpression inhibited the migration of all three cancer cell lines using Transwell assay(magnification, ×100). (C, D) The numbers of invading and metastasized cells were measured by Image J and represented by bar graph. (E) Gimap5 overexpression inhibited the proliferation of all three cancer cell lines using CCK-8 assay. The data were expressed as mean ± SEM (**p < 0.01 and ***p < 0.001 vs. empty vector) of the three independent experiments. (F) Gimap5 overexpression promoted apoptosis. After overexpression of Gimap5, the mRNA expression levels of caspase3 and caspase9 were up-regulated (*p < 0.05 vs. empty vector). After knockout of Gimap5, the mRNA expression levels of caspase3 and caspase9 were down-regulated (*p < 0.05 vs. shControl). (G) Flow cytometry showed that overexpression of Gimap5 significantly increased cell apoptosis in PC9 cells, however, there was no significant change in Beas-2b cells. In the scatter plot of the bivariate flow cytometer, the lower left quadrant shows living cells, which is (FITC-/PI-); The upper right quadrant is non-living cells, i.e. necrotic cells, and is (FITC+/PI+); The lower right quadrant is apoptotic cells, showing (FITC+/PI-).