Figure 5.

The interacted proteins of Gimap5 were screened using combined Co-IP and LC-MS/MS. (A) Silver staining map of Co-IP assay using Gimap5 antibody after transfection of Beas-2b or PC9 cells with Gimap5 overexpressed vector. The first lane represents marker; second lane is Co-IP using Beas-2b cells; the third lane is M6PR antibody to the Gimap5 IP products of the PC9 cells; the fourth lane is M6PR antibody to the Gimap5 IP products of the PC9-Gimap5 cells; the fifth lane is M6PR antibody to Gimap5 IP product of Beas-2b cells; and the sixth lane is M6PR antibody to the Gimap5 IP product of Beas-2b-Gimap5 cells. (B) Secondary peptide spectra of M6PR were detected in the Co-IP products using Gimap5 antibody. (C) the total protein of Beas-2b was extracted, and the interacting proteins of M6RP were screened using Co-IP. Western blot using anti-Gimap5 antibody showed that M6RP interacted with Gimap5. (D) The expression levels of M6PR in Co-IP products of Gimap5 and the expression levels of Gimap5 in Co-IP products of M6PR antibody were detected using Western blot. (E) The GO analysis of mass spectrometry of Gimap5 interacted proteins. (F) The GO analysis of mass spectrometry of M6PR interacted proteins.