FIGURE 4.

Trimetazidine inhibited the pressure overload-evoked myocardial hypertrophy and mitochondrial dysfunction in vitro. (A) Immunofluorescence staining of F-actin in NRCMs, nuclei were stained with DAPI, scale bar = 50 um. (B) NRCM area calculated from (A), each group included 10 cells. (C) The protein levels of ANP and BNP in NRCM and their quantitative data collected from four independent samples. (D) Representative ultrastructure mitochondrial image of NRCMs. scale bar = 0.5 um. (E) The ATP content of NRCMs (n = 4). (F,I) Representative western blots of mitochondrial dynamic protein and corresponding quantitative results. Graphical data are shown as mean ± SEM. Two-way ANOVA was used for analysis. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, vs indicated group. MS, Mechanical stretch; TMZ, Trimetazidine.