TABLE 2.
Specificities of primers against various target strains
| Strain | Amplification with the following primera:
|
|||||
|---|---|---|---|---|---|---|
| I | II | III | IV | Pg | U | |
| P. gingivalis | ||||||
| 381 | + | − | − | − | + | + |
| ATCC 33277 | + | − | − | − | + | + |
| BH18/10 | + | − | − | − | + | + |
| HW24D-1 | − | + | − | − | + | + |
| OMZ314 | − | + | − | − | + | + |
| OMZ409 | − | + | − | − | + | + |
| ATCC 49417 | − | − | + | − | + | + |
| 6/26 | − | − | + | − | + | + |
| HG564 | − | − | − | + | + | + |
| W50 | − | − | − | + | + | + |
| A. actinomycetemcomitans Y4 | − | − | − | − | − | + |
| F. nucleatum ATCC 10953 | − | − | − | − | − | + |
| P. intermedia ATCC 25611 | − | − | − | − | − | + |
| S. mutans MT8148 | − | − | − | − | − | + |
| S. sanguis ATCC 10556 | − | − | − | − | − | + |
| E. coli NM522 | − | − | − | − | − | + |
a
+, positive amplification; −, no PCR product; I to IV, fimA types; Pg, P. gingivalis species-specific 16S rRNA primers; U, universal primers for positive control.