Figure 5.

Characterization of whole mount scWAT nerves.

(A–G) scWAT depots from C57BL/6J mice were whole mount processed and stained with β3-Tubulin (green) and either TH (red) (A–C), AVIL (red) (D), or MPZ (red) (E–G). Whole mount tiled Z_max projections of ing-scWAT (A and D) and axi-scWAT (G) imaged with 10X objective. White boxes show a single tile expanded for visualization with 5.75X digital zoom (A and E). NB cross sections imaged with 63X objective with a 2.54X zoom factor applied to the digital cross-section rendering (B and E). Representative images of small fiber innervation imaged as Z_max projections with 63X objective (C and F). All images captured on Leica TCS SP8 DLS confocal microscope (A–G). Scale bars are 2 mm (A), 25 μm and 10 μm (B), 25 μm and 50 μm (C), 50 μm and 10 μm (D), 5 mm (E), 25 μm (F), a 25 μm and 10 μm (G).