Figure 4.

Hsa_circ_0024093 sponges miR-889-3p and miR-4677-3p to upregulate USP9X

(A and B) Subcellular fractionation and FISH assays (scale bar, 20 μm) monitored the cellular location of hsa_circ_0024093. (C) Enrichment of hsa_circ_0024093 and USP9X in anti-Ago2 in VSMCs and PDGF-BB-treated VSMCs was calculated. (D) The binding sites between hsa_circ_0024093 and miR-4677-3p/miR-889-3p were predicted on ENCORI. (E) Luciferase activity of hsa_circ_0024093-WT/MUT in indicated VSMCs with the transfection of miR-4677-3p mimics or miR-889-3p mimics was detected. (F) The binding sites of miR-889-3p/miR-4677-3p in the USP9X 3′ UTR were also predicted on ENCORI. (G) Luciferase activity of USP9X-WT/MUT in indicated VSMCs with overexpression of miR-889-3p or miR-4677-3p was detected. (H) Luciferase activity of USP9X-WT under different treatment and transfections was investigated. ∗∗p < 0.01; n.s., no significance.