Skip to main content
. 2021 Aug 19;26:557–574. doi: 10.1016/j.omtn.2021.08.013

Figure 2.

Figure 2

In vitro differentiation and senescence detection of MSCs at P4, P6, P8, P10, and P12

(A–C) Adipogenic differentiation (ORO staining) (A); osteogenic differentiation (ARS staining) (B); SA-β-gal staining (C) of MSCs at P4, P6, P8, P10, and P12. Scale bars, 50 μm in (A) and (C), 100 μm in (B). (D) Quantification of ORO staining in (A). (E) Quantification of ARS staining in (B). (F) Percent of SA-β-gal staining in (C). (G) Relative telomere length detection of MSCs in P4, P6, and P12. Data are presented as the means ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with P4. n = 9 MSCs per passage.