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. 2021 Aug 26;26:473–487. doi: 10.1016/j.omtn.2021.08.026

Figure 6.

Figure 6

circCD44 acts as a sponge and directly binds to miR-330-5p/miR-326

(A) RT-qPCR assays were conducted to investigate the impact of circCD44 on the expression of miRNAs that potentially bind to circCD44. (B) The putative binding sites of miR-326 and miR-330-5p on the circCD44 wild-type (WT) or mutated sequence are shown. (C) The luciferase reporter assay was performed to detect the activity of circCD44 in 1124C cells cotransfected with miR-326, miR-330-5p, or scramble and circCD44 WT or circCD44 mutated (MU) vector. (D) RNA pull-down was performed to detect the binding of circCD44 and miR-326 or miR-330-5p. (E) RNA pull-down was performed to detect the binding of circCD44 and biotinylated miR-326 (MU) or biotinylated miR-330-5p (MU). (F) Correlation between the expression of miR-326 and circCD44 in collected GBM samples was evaluated with Pearson’s correlation test. (G) Correlation between the expression of miR-330-5p and circCD44 in collected GBM samples was evaluated with Pearson’s correlation test.