Fig. 3.

(A) ASCs at passage 3 isolated from rats are positive for CD29, 73, 90, 105 and negative for CD34, 45. Blue histogram shows negative control. (B) After culturing passage 3 ASCs in each differentiation medium for 3 weeks, it was confirmed that the cells cultured in each medium had pluripotency into adipogenic, osteogenic, and chondrogenic differentiation by oil red O staining, alizarin red staining, and alcian blue staining. On the other hand, in control medium, ASCs did not have differentiation. The scale bar indicates 100 μm.