FIG. 3.
Effects of deletion of RPH1 and GIS1 on basal-level expression and damage induction of PHR1. Strains YPH499, GBS1734, GBS1736, and GBS1738 were transformed with a PHR1-lacZ reporter plasmid and with pRS415, pGBS712 (RPH1), or pGBS207 (GIS1), and the effect on expression was assessed with (cross-hatched bars) or without (open bars) MMS treatment. The chromosomal genotypes are indicated below the ordinate, and the induction ratio following MMS treatment is indicated immediately above the chromosomal genotype. Error bars show the standard deviations from three or four independent determinations. (A) Effects on expression from a reporter plasmid (pGBS116) that contains the intact PHR1 promoter. (B) Effect on expression of a pGBS116 derivative (pGBS759) in which the AG4 sequence has been mutated.