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. 2021 Sep 29;113:113–115. doi: 10.1016/j.ijid.2021.09.069

Figure 1.

Figure 1

(A) SARS-CoV-2 N antigen concentration in 257 samples positive to the Lumipulse® G SARS-CoV-2 Ag assay, grouped according to the number of genes resulted positive by RT-PCR: confirmed with complete set of genes, i.e. to 3 genes with the Allplex™ SARS-CoV-2 assay and to 2 genes with the Easy SARS-CoV-2 WE assay (median: 3.11 Log pg/mL); confirmed with incomplete set of genes, i.e. to 2 genes with the Allplex™ SARS-CoV-2 assay and to 1 gene with the Easy SARS-CoV-2 WE assay (median: 0.57 Log pg/mL); not confirmed (median: 0.28 Log pg/mL). Statistically significant differences in Student´s T test are indicated in the figure. (B) Correlation between Lumipulse Ag concentrations (Log pg/mL) and Ct values of RdRp gene from the RT-PCR combined results of Allplex™ SARS-CoV-2 Assay and Easy SARS-CoV-2 WE kit (n=513). (C and D) Correlation between Lumipulse Ag concentrations (Log pg/mL) and Ct values of RdRp gene split for from the Allplex™ SARS-CoV-2 Assay (n=387, C) and for the Easy SARS-CoV-2 WE kit (n=126, D). Samples with Ct <40 or pg/mL ≥1.34pg/mL were considered positive. For statistical calculations, an arbitrary value of 41 Ct was assigned to all RT-PCR negative samples and an arbitrary value of 1.0 pg/mL was assigned to all samples with Ag concentration <1.0 pg/mL.