Fig. 3.

A). Cross neutralization potential of SARS-CoV-2, HIV-1 polyclonal antibodies and effect of depletion on cross reactive binding antibodies on cross neutralization: Cross-neutralization potential of sera was assessed against pseudotyped viruses expressing HIV-1 clones representing different subtypes. The value represents the serum neutralization titers. In the case of SARS-CoV-2, a CPE based assay was performed and for HIV-1, a TZM-bl cell neutralization assay was carried out. Neutralization assays were done in duplicates and repeated at least two independent times B). The extent of binding of the depleted and undepleted spike serum with magnetic beads coated with HIV-1J41 gp140 protein was accessed by ELISA. C). Degree of shift in sensitivity of Wuhan SARS-CoV2 viruses to plasma depleted with HIV-1 Env (J41 gp140). The value in the graph represents the CPE value. No change in neutralization of plasma samples was observed in depleted and undepleted samples. Statistical significance was determined using student t-test and p < 0.05 was considered significant and ns (non-significant).