Fig. 4.

A & B). HIV-1 gp140 from 4-2J41 (clade C), JRFL (clade B) proteins were treated with PNGase and deglycosylated. The spike immunized mice sera bound with both glycosylated and deglycosylated forms of gp140 protein. In a parallel blot, an equal amount of proteins was loaded and Anti-His tag antibody was used for probing. C). Cross-reactivity of binding was assessed in presence of methyl α-d-mannopyranoside. Plates were coated with SARS-CoV-2 Spike protein and incubated with dilutions of methyl α-d-mannopyranoside along with a constant amount of the indicated antibodies. Antibody binding was quantified via ELISA. Statistical significance between the control and other experimental groups was estimated by two-way analysis of variance (ANOVA) followed by Tukey’s post hoc ergo propter hoc tests using GraphPad Prism 7. Statistical significance between control and different groups is shown as, ****P < 0.0001 and ns (non-significant).