Figure 3.
R-2HG-reduced ERα can suppress CCA cell growth via altering downstream gene YAP1 expression
(A and B) A western blot assay was performed to detect the four potential downstream genes that might be regulated by ERα in QBC939 cells w/wo ERα knockdown (A), and in HuCCT1 cells w/wo ERα overexpression (B). (C–E) QBC939 cells were transfected w/wo shERα and subsequently transfected w/wo oeYAP1, after which an MTT assay (C) and colony formation (D) were performed to examine cell growth. A western blot assay (E) was used to reveal ERα and YAP1 expressions. (F–H) HuCCT1 cells were transfected w/wo oeERα and subsequently transfected w/wo shYAP1, after which an MTT assay (F) and colony formation (G) were performed to examine cell proliferation, and ERα and YAP1 expressions were detected by a western blot assay (H). (I–K) The results of an MTT assay (I) and colony formation (J) demonstrate the efficiency of cell growth in QBC939 cells w/wo IDH1R132H and transfected w/wo oeYAP1, and ERα and YAP1 expressions were detected by a western blot assay (K) . For (D), (G), and (J), quantitations are at the right and presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01; NS, not significant.