Skip to main content
. 2021 Aug 18;62:100108. doi: 10.1016/j.jlr.2021.100108

Fig. 2.

Fig. 2

Glucose-stimulated insulin release from MIN6 cells. Many FAHFA isomers potentiate glucose-stimulated insulin secretion in clonal pancreatic β-cells, MIN6 cells. MIN6 cells were incubated with DMSO (0.1%, control) or FAHFAs (20 μM) for 1 h and then stimulated with low (2.5 mM) or high (20 mM) concentrations of glucose for 45 min. n = 6–8 wells/condition. Data are the means ± SEM. Each isomer was tested in 2–3 independent studies. ∗P < 0.05 versus DMSO with 20 mM glucose; one-way ANOVA. A: 5-, 9-, 10-, 12-, and 13-PAHSA, palmitic acid (PA), and 9-hydroxy stearic acid (HSA); (B) 5-, 9-, 10-, 12-, and 13-POHSA; (C) 5-, 9-, 10-, 12-, and 13-OAHSA; (D) 5-, 9-, 10-, 12-, and 13-SAHSA; (E) palmitic acid, palmitoleic acid (PO), oleic acid (OA), stearic acid (SA), and hydroxy stearic acids (5-, 9-, and 12-HSA). FAHFA, fatty acid ester of hydroxy fatty acid; PAHSA, palmitic acid hydroxy stearic acid; POHSA, palmitoleic acid hydroxy stearic acid; OAHSA, oleic acid hydroxy stearic acid; SAHSA, stearic acid hydroxy stearic acid.