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. 2021 Aug 18;62:100108. doi: 10.1016/j.jlr.2021.100108

Fig. 4.

Fig. 4

HEK293T mouse GPR40 FLIPR, calcium assay agonist mode with FAHFAs. Many but not all FAHFA isomers activate mouse GPR40 in HEK293T cells. Activation of mouse GPR40 in HEK293T cells by various FAHFA isomers (A). Data are the means ± SEM. n = 4–6; ∗P < 0.05 versus buffer; one-way ANOVA. B: Comparison of GPR40 activation (at 50 or 100 μM) in HEK293T cells with GSIS (at 20 μM) in human islets and clonal pancreatic β-cells, MIN6 cells. C: GPR40 is not activated by palmitic acid, stearic acid, or 5-hydroxy stearic acid in HEK293T. Data are the means ± SEM n = 4. FAHFA, fatty acid ester of hydroxy fatty acid; GPR40, G protein–coupled receptor 40; GSIS, glucose-stimulated insulin secretion.