REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal 910-30	This paper	N/A
Monoclonal 1-20	Ho Lab	Liu et al. 2020a
Monoclonal B38	Ho Lab	Wu et al., 2020b
Monoclonal C105	Bjorkman Lab	Barnes et al., 2020
Monoclonal CR3022	Neil King and David Veesler at the University of Washington	RRID: AB_2848080
Goat anti-Human IgG Fc PE	Invitrogen	Cat #12-4998-82; RRID: AB_465926
Monoclonal Anti-MYC-FITC	Sigma-Aldrich	Cat #SAB4700448; RRID: AB_10896411
Monoclonal Anti-FLAG-FITC	Sigma-Aldrich	Cat #F4049; RRID: AB_439701
Bacterial and virus strains
High-efficiency electrocompetent E. coli	New England Biolabs	Cat #C2987H
Authentic SARS-CoV-2	Ho Lab	Ho et al., 2020
AWY101 (MATα AGA1::GAL1-AGA1::URA3 PDI1::GAPDH-PDI1::LEU2 ura3-52 trp1 leu2Δ1 his3Δ200 pep4::HIS3 prb1Δ1.6R can1 GAL)	Eric Shusta Lab, University of Wisconsin-Madison	N/A
Biological samples
Plasma from SARS-CoV-2 patients	Hong Kong University	N/A
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 RBD	Vaccine Research Center	Zhou et al., 2020c
SARS-CoV-2 S2P	Vaccine Research Center	Zhou et al., 2020c
Dna Clean & Concentrator Kits, Zymo Research	Zymo research	Cat #D4005
Expi293 Expression Medium	GIBCO	Cat #A1435102
EasySep Human B cell enrichment kit w/o CD43 depletion	STEMCELL Technologies	Cat #17963
Monarch DNA Gel Extraction Kit	NEB	Cat #T1020
NEBuilder HiFi DNA	NEB	Cat #E5520
SDCAA medium	Teknova	Cat#2S0540
FuGENE 6	Promega	Cat #E2693
ACE2-Fc	Neil King and David Veesler at the University of Washington.	N/A
NHS-Ester biotin	ThermoFisher EZLink Biotin	Cat #20217
YPD medium	ThermoFisher	Cat #242820
Frozen-EZ Yeast Transformation II kit	Zymo Research	Cat #T2001
Human CD27 Microbeads	Miltenyi Biotec	Cat #130-051-601
SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase	Thermo Fisher Scientific	Cat #12574026
Deposited data
Sequence data	GenBank	MY291105
Sequence data	GenBank	MY291106
Structures	PDB	7KS9
Electron microscopy data	EMDB	EMD-23016
Electron microscopy data	EMDB	EMD-23039
Supplemental tables	Mendeley	https://doi.org/10.17632/6rtg8t48zk.1
Experimental models: cell lines
Expi293F human cells	GIBCO	Cat #A14527
Irradiated 3T3-CD40L fibroblast	Gift from John Mascola, Vaccine Research Center, NIAID	N/A
Vero cells	ATCC	Cat #CRL-1586
HEK293T	Ho Lab	Ho et al., 2020
Oligonucleotides
VH:VL amplification and cloning primers	IDT	McDaniel et al., 2016
VH:VL amplification and cloning primers	IDT	Wang et al., 2018
Recombinant DNA
pCT-VHVL-K1	NIAID	Wang et al., 2018
BRS-hIgG1	NIAID	Wang et al., 2018
EVB 114 kc	NIAID	Wang et al., 2018
EVB100_Lc	NIAID	Wang et al., 2018
pCMV3-SARS-CoV-2-spike	Sino Biological	N/A
Software and algorithms
Usearch	USEARCH	https://www.drive5.com/usearch/
MiXCR	MiLaboratory, LLC	https://mixcr.readthedocs.io/en/master/
Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/; RRID: SCR_002798
R	The R Foundation	https://cran.r-project.org
tidyverse	R studio	https://www.tidyverse.org/