Table II.
The primer information.
| Gene names | Primer direction | Sequence (5' - 3') | Tm(°C) | Location | Amplification size |
|---|---|---|---|---|---|
| COL1A1 | Forward | GAGGGCCAAGACGAAGACATC | 62.5 | 85 to 105 | 140 |
| Reverse | CAGATCACGTCATCGCACAAC | 61.6 | 224 to 204 | ||
| COL3A1 | Forward | GGAGCTGGCTACTTCTCGC | 62.2 | 23 to 41 | 78 |
| Reverse | GGGAACATCCTCCTTCAACAG | 60 | 100 to 80 | ||
| MMP1 | Forward | AAAATTACACGCCAGATTTGCC | 60 | 356 to 377 | 82 |
| Reverse | GGTGTGACATTACTCCAGAGTTG | 60.5 | 437 to 415 | ||
| MMP3 | Forward | AGTCTTCCAATCCTACTGTTGCT | 61 | 7 to 29 | 226 |
| Reverse | TCCCCGTCACCTCCAATCC | 63 | 232 to 214 | ||
| MMP9 | Forward | TGTACCGCTATGGTTACACTCG | 61.5 | 146 to 167 | 97 |
| Reverse | GGCAGGGACAGTTGCTTCT | 61.9 | 242 to 224 | ||
| MMP13 | Forward | ACTGAGAGGCTCCGAGAAATG | 61.3 | 193 to 213 | 103 |
| Reverse | GAACCCCGCATCTTGGCTT | 62.7 | 295 to 277 | ||
| TGF-β1 | Forward | GGCCAGATCCTGTCCAAGC | 62.4 | 151 to 169 | 201 |
| Reverse | GTGGGTTTCCACCATTAGCAC | 61.2 | 351 to 331 | ||
| TGF-β2 | Forward | CAGCACACTCGATATGGACCA | 61.6 | 72 to 92 | 113 |
| Reverse | CCTCGGGCTCAGGATAGTCT | 62.1 | 184 to 165 | ||
| SMAD3 | Forward | TGGACGCAGGTTCTCCAAAC | 62.4 | 284 to 303 | 90 |
| Reverse | CCGGCTCGCAGTAGGTAAC | 62.1 | 373 to 355 | ||
| WNT11 | Forward | GGAGTCGGCCTTCGTGTATG | 62.3 | 327 to 346 | 167 |
| Reverse | GCCCGTAGCTGAGGTTGTC | 62.4 | 493 to 475 | ||
| CCNB1 | Forward | TTGGGGACATTGGTAACAAAGTC | 60.7 | 134 to 156 | 226 |
| Reverse | ATAGGCTCAGGCGAAAGTTTTT | 60.2 | 359 to 338 | ||
| CCNE2 | Forward | TCAAGACGAAGTAGCCGTTTAC | 60 | 4 to 25 | 115 |
| Reverse | TGACATCCTGGGTAGTTTTCCTC | 61.3 | 118 to 96 | ||
| CCNA2 | Forward | CGCTGGCGGTACTGAAGTC | 62.7 | 146 to 164 | 120 |
| Reverse | GAGGAACGGTGACATGCTCAT | 62.1 | 265 to 245 | ||
| CDK1 | Forward | AAACTACAGGTCAAGTGGTAGCC | 61.8 | 71 to 93 | 148 |
| Reverse | TCCTGCATAAGCACATCCTGA | 60.9 | 218 to 198 | ||
| E2F1 | Forward | ACGCTATGAGACCTCACTGAA | 60.3 | 378 to 398 | 249 |
| Reverse | TCCTGGGTCAACCCCTCAAG | 63 | 626 to 607 | ||
| CDC6 | Forward | CCAGGCACAGGCTACAATCAG | 62.8 | 18 to 38 | 116 |
| Reverse | AACAGGTTACGGTTTGGACATT | 60.1 | 133 to 112 | ||
| CDC7 | Forward | GAGGCGTCTTTGGGGATTCAG | 62.7 | 4 to 24 | 245 |
| Reverse | GGTCCTACTTGTAACTGTGCTG | 60.3 | 248 to 227 | ||
| ACTB | Forward | GGCTGTATTCCCCTCCATCG | 61.8 | 84 to 103 | 154 |
| Reverse | CCAGTTGGTAACAATGCCATGT | 61.1 | 237 to 216 |
ACTB, actin beta; CCNB1, Cyclin B1; CDC6, cell division cycle 6; CDK1, cyclin dependent kinase 1; COL1A1, collagen, type 1, alpha 1; COL3A1, collagen, type 3, alpha 1; MMP, matrix metalloproteinase; SMAD3, Mothers against decapentaplegic homolog 3; TGF-β1, tumour growth factor beta 1.