Figure 7.

Differences in dendritic spines and microglial morphology between GF and SPF mice

(A) Representative images of Imaris 3D reconstruction for pyramidal neuronal spine density analysis from cortex. White line is 5 μm. SPF (above) and GF (below).

(B) Spine density per 10 μm of dendrite and spine length in the ipsilateral hemisphere of GF and SPF mice 28 d after ischemic stroke (Student's t test, p < 0.05 and p < 0.001; n(SPF) = 7; n(GF):10).

(C) Representative image of Iba1 immunostaining (red) with counterstained cell nuclei (blue) (left) and the binary mask for microglia staining with Iba1 (right).

(D) Circularity index for microglia in naive GF and SPF mice as well as 28 d post-stroke. n(naive) = 5 mice per group; n(stroke) = 7 mice per group, Student's t test, p < 0.05 and p < 0.001).