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. Author manuscript; available in PMC: 2022 Mar 8.
Published in final edited form as: Biomacromolecules. 2021 Feb 8;22(3):1065–1079. doi: 10.1021/acs.biomac.0c01085

Figure 3.

Figure 3.

(a) Schematic of experimental design to test for efficacy of immobilizing BMP2-SH in hydrogel using SMAD 1/5/8 reporter cells and a modified-ELISA. Hydrogels were formed from a precursor solution containing no BMP-2, naive BMP-2, or BMP2-SH and then photopolymerized. The precursor solution contained 8-arm PEG-norbornene, PEG-dithiol, and photoiniator (I2959). Once formed, the hydrogels were swelled in cell-culture media for 24 hours to release any untethered BMP-2 or BMP2-SH. Luciferase reporter cells were treated with released BMP. (b) Luminescence of the reporter cells as a function of BMP-2 and BMP2-SH released from hydrogels. Data are normalized to the no BMP-2 condition. (n = 4). Data were analyzed using a one-way ANOVA and a Bonferroni’s post-hoc comparison test.(c) Absorbance measured from a modified ELISA quantifies the presence of tethered BMP2-SH on the surface of the PEG hydrogel (n = 3). Data represent average with standard deviation error bars.