Table 2.
Nomenclature | BMP-2 (nM)1 | Tethered BMP-2 (nM)2 | Tethered BMP-2 (ng mL−1)2 |
---|---|---|---|
10 nM BMP2-SH | 10 | 4 | 100 |
100 nM BMP2-SH | 100 | 40 | 1000 |
500 nM BMP2-SH | 500 | 200 | 5000 |
Concentration of BMP-2 used in the thiolation reaction.
Estimated concentration of tethered BMP-2 concentration in the gel after thiolation and immobilization based on modified ELISA, Figure 3c.