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. 2021 Mar 11;93(11):4859–4866. doi: 10.1021/acs.analchem.0c04731

Figure 2.

Figure 2

(A) Calibration curves of the As385/HHQ-BSA ELISA for the detection of the PQS run in buffer (PBST-EDTA) and in 1/10 diluted MH broth, under the conditions established (see Table 1). Each calibration point was measured in triplicates on the same ELISA plate, and the results show the average and standard deviation of analysis made on three different days. (B) Matrix effect of MH broth undiluted and diluted 2, 5, 10, and 20 times with PBST on the As385/HHQ-BSA ELISA. The calibration curves were run using the conditions established for the assay in PBST. Modification of the assay conditions allowed us to achieve similar immunoassay features as when the assay was run in buffer (see Table 1 and panel A). The results shown are the average and standard deviations of analysis made on two different days measured by duplicates each day.