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Solvatochromic trehalose probes label the mycobacterial mycomembrane. (A) Solvatochromic trehalose probes are converted by mycobacteria to the corresponding trehalose monomycolate (TMM, structure on right) analogs and inserted into the mycomembrane. There, they undergo fluorescence turn-on, enabling detection of labeled cells by fluorescence microscopy. (B) Chemical structures of solvatochromic dyes described in this study.
