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. 2021 Jul 26;1(9):1368–1379. doi: 10.1021/jacsau.1c00173

Figure 6.

Figure 6

Unlike 3HC-2 dye conjugates, 3HC-3-Tre labeling is initially localized at the septum and poles. (A) Time-lapse microscopy of Msmeg cells treated with 100 μM DMN-Tre, 3HC-3-Tre, 3HC-2-Tre, or 3HC-2-Glc for 30 min revealed concentration of 3HC-3-Tre at cell septa and poles. White arrows denote septal labeling. Scale bar: 5 μm. (B) Quantification of Msmeg fluorescence in the presence of 100 μM (i) DMN-Tre, (ii) 3HC-3-Tre, or (iii) 3HC-2-Tre during labeling for 30 min (left, volume-normalized intensity) and subsequent washing with growth medium for 1 h (right, total fluorescence). The number of cells included in each analysis (n) is provided in each panel. Shaded error bars represent ±1 standard deviation.