FIG. 4.

Western immunoblot reacted with anti-E. chaffeensis HMAF showing the proteins expressed by three different size variations of the VLPT gene. The lanes contained lysates from purified E. chaffeensis Arkansas (lane Ec) E. coli transformed with a VLPT clone selected from the E. chaffeensis Arkansas genomic library (lane Ac), and E. coli transformed with plasmids containing PCR amplicons from the following strains: St. Vincent (lane 3R), Jax (lane 4R), and 91HE17 (lane 5R) (R indicates the number of 90-bp repeat units found in the VLPT gene of each strain).