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. 2021 Sep 15;12:725734. doi: 10.3389/fphar.2021.725734

TABLE 1.

Erythropoietin regulation of fat mass and inflammation in mice during diet induced obesity.

Mouse model EPO status Physiologic response Physiologic response References
WT (C57BL/6) EPO gene electrotransfer in skeletal muscle Fat mass -EPO in females on high fat diet (HFD) decreased body weight/fat mass and improved glucose tolerance Hojman et al. (2009)
EPO treatment Fat mass -EPO decreased blood glucose (also WT BALB/c) Katz et al. (2010)
EPO treatment Fat mass -In males on HFD, EPO ≥ 150 U/kg reduced body weight/fat mass, ≥ 300 improved glucose tolerance, at 1000 U/kg increased physical activity Foskett et al. (2011)
EPO treatment Fat mass -EPO in males reduced body weight/fat mass accumulation on HFD and reduced body weight on NCD Teng et al. (2011)
EPO treatment Fat mass -EPO Increased WAT metabolic activity, mitochondria content, oxygen consumption, brown fat program in males Wang et al. (2013)
EPO treatment WAT inflammation -EPO reduced obese WAT inflammation, inflammatory cytokine production, macrophage infiltration, shifted WAT macrophages to anti-inflammatory phenotype in males Alnaeeli et al. (2014)
EPO treatment Fat mass -EPO reduced body weight, fat mass and activated brown adipose tissue during HFD. Kodo et al. (2017)
EPO treatment Fat mass -EPO reduced body weight/fat mass accumulation during NCD and HFD in males. Zhang et al. (2017)
-EPO did not change body weight/fat mass in females
-EPO reduced body weight/fat mass accumulation in ovariectomized females on HFD.
EPO treatment Bone marrow adipose tissue -EPO reduced bone marrow adipocytes and bone without change in WAT. Suresh et al. (2019)
EPO treatment Bone marrow adipose tissue -EPO reduced HFD increase in bone marrow adipocytes independent of change in whole fat mass in males and females Suresh et al. (2020c)
Brain EPO administration Fat mass Hypothalamus inflammation -EPO reduced body weight/fat mass accumulation and hypothalamus inflammation during HFD in males Dey et al. (2020)
-EPO did not change body weight/fat mass accumulation and hypothalamus inflammation during HFD in females
-EPO reduced body weight/fat mass accumulation and hypothalamus inflammation during HFD in ovariectomized females
Brain EPO administration Fat mass -EPO decreased body weight/fat mass, increase lean mass, reduced food intake in males Wang et al. (2020)
tg6 (C57BL/6) High transgenic human EPO Fat mass -Males and females have lower body mass Katz et al. (2010)
Bone marrow adipose tissue -Males and females have reduced bone marrow adipocytes and bone Suresh et al. (2019)
tg21 (C57BL/6) High brain transgenic human EPO Fat mass Hypothalamus inflammation -Males have reduced body weight/fat mass accumulation and hypothalamus inflammation during HFD Dey et al. (2020)
-No change in body weight/fat mass accumulation and hypothalamus inflammation during HFD in females
-Ovariectomized females have reduced body weight/fat mass accumulation and hypothalamus inflammation during HFD
ΔEPORE (C57BL/6) EPOR restricted to erythroid tissue Fat mass -Males and females have increased fat mass, glucose intolerance and insulin resistance. Teng et al. (2011)
Bone marrow adipose tissue -Males and females have reduced trabecular bone and increase bone marrow adipocytes that decreases with EPO treatment. Suresh et al. (2019)
-EPO reduced bone marrow adipocytes without change in WAT
WAT inflammation -Increased WAT inflammation in obese ΔEpoRE mice. Alnaeeli et al. (2014)
EPOR(aP2KO) (C57BL/6) EPORloxP/loxP ;aP2-Cre fat knockout Fat mass -Males have increased susceptibility to diet induced obesity Wang et al. (2013)
EPOR(aP2KO) (129J-C57BL/6-FVB/N) EPORloxP/loxP ;aP2-Cre fat knockout Body weight -Body weight unchanged on NCD or HFD Luk et al. (2013)
WAT Inflammation -WAT inflammation unchanged
EPOR(nestinKO) (C57BL/6) EPORloxP/loxP ;nestin-Cre neural knockout Fat mass -Increased inflammation and weight gain during diet induced obesity in males and not females Dey et al. (2020)
Hypothalamus inflammation
Ob/ob (C57BL/6) Obesity model Body weight -In males, EPO reduced body weight Katz et al. (2010)
Fat mass -EPO reduced body weight/fat mass accumulation Teng et al. (2011)
PTP1B−/− (BALB/c) Obesity model Body weight -In males, EPO reduced body weight gain, Hb A1c Katz et al. (2010)
STAT6−/− (C57BL/6) Defective anti-inflammatory like macrophages WAT inflammation -In obese males, EPO does not shift WAT macrophages to anti-inflammatory phenotype observed in obese WT Alnaeeli et al. (2014)
IL4−/− (C57BL/6)
ERα−/− (C57BL/6) Estrogen receptor α knockout Fat mass -EPO reduced HFD body weight/fat mass accumulation in WT males, in ERα−/− males and females Lee et al. (2021)
ERα(adipoKO) (C57BL/6) ERαloxP/loxP ;adiponectin-Cre fat knockout Fat mass -EPO reduced HFD body weight/fat in ERα(adipoKO) females Lee et al. (2021)
WAT inflammation -EPO reduced obese WAT inflammation in ERα(adipoKO) females but not in WT females